Yeast Culture Lab

In: Science

Submitted By amymo
Words 1353
Pages 6
Yeast Culture Lab

Introduction Yeast is a one-celled, microscopic organism, which is part of the fungi kingdom. Yeasts do not make up a single group (Smith & Smith, 2012). Yeasts use organic material as a means of making energy, which make them chemoorganotrophs (Smith & Smith, 2012). Carbon is procured primarily from hexose sugars, such as fructose and glucose. Yeast need either oxygen for aerobic cellular respiration or for species that are anaerobic, but also have aerobic methods creating energy (Smith & Smith, 2012). There are no species of yeast species that are known to grow only anaerobically. Yeasts thrive in an environment with a slightly acidic (Smith & Smith, 2012). The reproductive cycle of yeasts can be either asexual or sexual depending on the species. The most widely seen method of growth in yeast is asexual reproduction referred to as budding (Smith & Smith, 2012). Reproduction in reference to yeast depends on the species; the species can be both asexual by mitosis and sexual by budding (Smith & Smith, 2012). Consumption refers to use and the rate of use of something such as how a consumer, such as a primary consumer like a tree would use photosynthesis to make energy from carbon dioxide. Death in reference to a population is referring to the rate of death in that population (Smith & Smith, 2012).
The primary goal of the yeast culture lab is to test a theory involving samples of yeast cultures grown in four conditions. The initial hypothesis is that yeast will undergo considerable growth in all environments but will show an increased rate of growth in a solution of sugar because yeast uses sugar as a source to generate energy naturally. The assumption is that this hypothesis is correct and that the yeast will grow quickly in each environment and,in particular, in the sugar solution.…...

Similar Documents

The Effect F of Different Type of Sugar on Yeast

...The Effect of Different Types of Sugar on Respiration in Yeast INTRODUCTION: Yeast is living bacteria that uses in baking to make dough rise through the process of fermentation. For fermentation to occur yeast requires fuel in the form of sugar. The yeast reaction varies depending upon the type of sugar use. AIM: To determine the effect of different types of sugar (Maltose, Lactose and Glucose) have on the respiration rate of yeast. HYPOTHESIS If the type of sugar changed (Maltose, Lactose and Glucose), the amount of carbon dioxide created (bubbles) will then increase because sugar needed for respiration to occur. But, I think Maltose + Yeast will produce more bubbles than other two types of sugar because maltose is the largest sugar in all types of sugar which I am going to do on. Glucose will come into a second place and the least will be Lactose. VARIABLES * INDEPENDENT VARIABLE The independent is type of sugar. I will change it by putting different types of sugar into the water which the yeast would be placed in. * DEPENTDENT VARIABLE The dependent is the amount of bubbles collected from the over a certain amount of time. Rate = CO2/Time * CONTROL VARIABLE 1. Amount of yeast(5 grams) 2. Temperature (50C) 3. Amount of time (1 minute) 4. Amount of sugar (100ml) 5. Concentration (5%) MATERIALS: -Yeast (5 grams) -Sugar (Maltose, Lactose and Glucose 100ml each) -Hot water (50C) -Water bath -Beaker (2) -Delivery......

Words: 1069 - Pages: 5

Yeast Fermentation

...Yeast Fermentation Copyright © 2000 Sarah Phillips Sarah Phillips, Inc. All rights reserved. Yeast is the most commonly used leavener in bread baking and the secret to great bread making lies in its fermentation, or the metabolic action of yeast. It is the magical process that allows a dense mass of dough to become a well-risen and flavorful loaf of bread. In order for fermentation to take place, all yeast needs food, moisture and a controlled warm environment. Its byproducts from consuming food are the gas carbon dioxide, alcohol, and other organic compounds. The gas is the rising agent in bread, and the other "waste" products create the subtle flavors and texture that make a good loaf. HOW DOES IT WORK? In bread baking, we are trying to ferment grain in order to leaven it. We are also trying to release sugars trapped in the complex starch molecules to be used as yeast food, and much of it for flavor and crust color (caramelization). Yeast is a single-celled organism and only certain strains are used for fermenting grain. Yeast activation and the initiation of fermentation are triggered by hydration, from either water or some other liquid, and the presence of a food source. Fermentation ends at 140 degree F during baking when heat kills the yeast. (Fermentation can end earlier, if the yeast is killed by other factors.) Yeast feeds on sugar derived from the complex starch molecules from flour, a complex carbohydrate. The starch molecules are broken apart into......

Words: 1963 - Pages: 8


...and Code of Conduct Preface LAB #1 ix vii Perform Reconnaissance and Probing Using Zenmap GUI (Nmap) Introduction Deliverables Hands-On Steps 1 1 3 14 15 2 Learning Objectives 1 Evaluation Criteria and Rubrics LAB #1 AssessmeNt WoRksheet LAB #2 Perform a Vulnerability Assessment scan Using Nessus Introduction Deliverables Hands-On Steps 19 19 21 31 32 20 Learning Objectives 19 Evaluation Criteria and Rubrics LAB #2 AssessmeNt WoRksheet LAB #3 enable Windows Active Directory and User Access Controls Introduction Deliverables Hands-On Steps 35 35 37 49 50 36 Learning Objectives 35 Evaluation Criteria and Rubrics LAB #3 AssessmeNt WoRksheet LAB #4 Configure Group Policy objects and microsoft® Baseline security Analyzer (mBsA) Introduction Deliverables Hands-On Steps 53 53 55 63 64 54 Learning Objectives 53 Evaluation Criteria and Rubrics LAB #4 AssessmeNt WoRksheet iii 38351_FMxx_Pass4.indd 3 23/04/13 5:13 AM iv Contents LAB #5 Perform Protocol Capture and Analysis Using Wireshark and NetWitness Investigator 67 Introduction Deliverables Hands-On Steps 67 67 69 80 81 68 Learning Objectives Evaluation Criteria and Rubrics LAB #5 AssessmeNt WoRksheet LAB #6 Perform Business Continuity Implementation Planning Introduction Deliverables Hands-On Steps 85 85 86 87 90 91 Learning Objectives and Outcomes 85 Evaluation Criteria and Rubrics LAB #6 AssessmeNt WoRksheet LAB #7 Relate Windows......

Words: 26285 - Pages: 106


...Change: * Constantly adapting (Obama Care) * Healthcare changes constantly (vascular lab yrs ago never had duplex. Nvr MRI…) * Cost change is the MOST CHANGE (motivated by cost) COST: * Medicare * Senor Citizens (over age of 65) * Dylysis * Medicaid * State funded * Pays 30 cents on the dollar to hospitals. (So hospital practically giving free help) * Insurance has change * Increase in specialties * Changes in doctor fees * Most hospitals can’t afford all things needed for patients (MRI, UT, nuclear labs…) * Increase in Pay for workers in Hospital * We don’t want to lower care to patients also saving money. Control costs) * (HMO- need referrals to see specialist) * Hospitals lowered # of patients beds. A lot more outpatient surgeries * Most concerns relate to costs and access Capitated Payment= business deals with insurance company for their employees. We have x number of employees and we will pay you a 1,000 dollars for you to take care of our employees. Insurance wants the most money for the time. (will make people come and talk abt how to be healthy so the employees don’t go to hospital which in turns makes more money for them. Payment based on performance: Doctors keep number of referrals down and keep a certain number of patients a day. If they keep this up they get a bonus. Implementation of clinical practice guidelines: Strict guidelines to get......

Words: 1302 - Pages: 6

Yeast Culture Lab

...Yeast Culture Lab BIO/315 April 22, 2014 Annissa Furr Yeast Lab Yeast, is a microscopic one celled organism that belongs to the group of organisms that is called fungi. They are single celled organisms that have a scientific name of Saccharomyces cerevisiae. Yeast can have many purposes but the main purpose of yeast is to help the fermentation process. Yeast is a living organism known as a fungus and it gets its energy source from sugar. Yeast can also be used in creating certain medical intentions that heal wounds and reduce inflammation because of the large amount of hormones and enzymes. In reference to yeast reproduction depends on the type of species because they can be either asexual by mitosis or sexual by budding. Consumption talked about the use and rate of use of a primary consumer that needs photosynthesis in order to create energy from CO2. Death is in reference to a population and refers to the toll of death in a population. Hypothesis This labs main objective is to test cultures referring to yeast. The hypothesis is that the yeast will grow in all the environments that it is placed in but it will thrive in the environment that has sugar that is because yeast uses sugar as an energy sources naturally which will make the yeast generate more. The yeast could be identical in all the environments but most likely the situation will be that the yeast grows rapidly in the sugar. This hypothesis needs to be tested and in order to do that their...

Words: 1280 - Pages: 6

Bio156 Lab 5

...56 Define the following terms: Cellular respiration (aerobic respiration) (2 points) Cellular respiration is the process by which cells get their energy in the form of ATP. There are two types of cellular respiration, aerobic and anaerobic. Aerobic respiration is more efficient and can be used in the presence of oxygen. Aerobic respiration, or cell respiration using oxygen, uses the end product of glycolysis in the TCA cycle to produce more energy currency in the form of ATP than can be obtained from an anaerobic pathway. Fermentation (anaerobic respiration) (2 points) Fermentation is a metabolic process converting sugar to acids, gases or alcohol.  It occurs in yeast and bacteria, but also in oxygen-starved human muscle cells. Fermentation is used by humans to make beer and food, like kimchi. Fermentation is a form of anaerobic digestion that generates ATP by the process of substrate-level phosphorylation. Summarize what occurs during the three steps of cellular respiration and indicate where each process takes place in the cell. (6 points) Glycolysis: It is a process that occurs in the cytoplasm. It converts each molecule of glucose to two molecules of pyruvic acid. It refers to an anaerobic process that proceeds whether or not oxygen is present or not. The pyruvic acid diffuses into the inner compartment of the mitochondrion where a transition reaction  occurs that starts to prepare pyruvic acid for the next stage of respiration. Krebs cycle-This......

Words: 964 - Pages: 4


...Tissues The Integumentary System 35 43 The Cell: Transport Mechanisms and Permeability–Wet Lab Classification of Covering and Lining Membranes 49 Overview of the Skeleton: Classification and Structure of Bones and Cartilages 53 The Axial Skeleton The Fetal Skeleton 59 67 81 87 93 103 77 The Appendicular Skeleton Articulations and Body Movements Microscopic Anatomy and Organization of Skeletal Muscle Gross Anatomy of the Muscular System Histology of Nervous Tissue 113 119 125 Exercise 16A Skeletal Muscle Physiology: Frogs and Human Subjects Exercise 18A Neurophysiology of Nerve Impulses: Wet Lab Gross Anatomy of the Brain and Cranial Nerves Electroencephalography Human Reflex Physiology General Sensation 153 157 167 177 173 Special Senses: Vision 133 Spinal Cord, Spinal Nerves, and the Autonomic Nervous System 145 137 Special Senses: Hearing and Equilibrium Special Senses: Olfaction and Taste Functional Anatomy of the Endocrine Glands iii Exercise 28A Role of Thyroid Hormone, Pituitary Hormone, Insulin, and Epinephrine: Wet Lab 183 Exercise 29A Blood Exercise 30 Exercise 31 Exercise 32 189 199 205 209 Anatomy of the Heart Conduction System of the Heart and Electrocardiography Anatomy of Blood Vessels Exercise 33A Human Cardiovascular Physiology: Blood Pressure and Pulse Determinations 217 Exercise 34A Frog Cardiovascular Physiology: Wet Lab Exercise 36 Exercise 38 Exercise 40 Exercise 42 Exercise 43 Exercise 44 Exercise 45 Exercise......

Words: 120457 - Pages: 482

1930's Culture Lab Activity

...1930’s Culture – Lab Activity Famous movies FYI Snow White – first full length animated film Gone With The Wind – first Technicolor film – 1400 girls auditioned for Scarlet O’Hara, and Vivian Leigh won the role The Wizard of Oz – Judy Garland Mickey Mouse; King Kong Go to a) Search for Hindenburg. Watch the 1:12 video of this disaster. What happened? The world’s largest aircraft burned up in flames. b) Search for Shirley Temple The Good Ship Lollipop. Watch a minute. Why was she among the most beloved child stars of all time? (Later a U.S. ambassador.) She among the most beloved child stars of all time because she helped the adults forget the trouble they were going through. c) Search for Swing Time Rogers and Astaire. Fred Astaire is still considered to be one of the greatest dancers of all time. Why do you think some people hold his ability in such high regard (what was special about it)? I think some people hold his ability in such high regard because his dance was different from what people were used to . d) Search for Gone With The Wind trailer. What main things was this 4 hour classic film about? It was about a love story. e) Search for Billie Holiday “One for my baby (and one more for the road)”. What were some vocal qualities that she possessed that you think made her so popular? Some vocal qualities that she possessed that I think made her so popular was her ability to relax people......

Words: 579 - Pages: 3


...Game Based Learning Zainal A. Hasibuan, Yugo K.Isal, Baginda Anggun N.C., Mubarik Ahmad, and Nungki Selviandro phase to implementation phase. Next, experimental games to study the influence of game culture on students. Finally, the conclusions of the article and future research. Abstract—Indonesia is a country consisting of thousands of islan. With many islands in Indonesia then there are many cultures of various ethnic groups scattered across Indonesia. With the cultural diversity that exist in Indonesia, then the existing culture must be preserved because the culture is a very important element in representing the personality of a country. There is a tendency that Indonesian young generation do not recognize their cultures. "Desa Maya Budaya Indonesia" (DayaBaya) : Virtual Village with Indonesian Culture - is a game developed to introduce Indonesian culture to young generation. This game simulates a virtual village located in a region in Indonesia that can be developed according to the player’s desires and wishes. The simulation will help young generation to know various cultures of Indonesia at the early stage. This game has also been tested on 48 students from Vocational High School,and a result from this experiment shows that the game improves user’s knowledge about cultures. II. PREVIOUS STUDY In the previous studies, the DayaBaya game has been developed. In this section, the game for education, game plan, game design and game development will be explained. A. Game......

Words: 3417 - Pages: 14


...Introduction: Yeast cells are simple, unicellular, eucaryotic organisms belonging to the Fungi kingdom. These cells are particularly important as tools for research because they share structural and compositional similarity with cells of higher organisms. This experiment makes use of these similarities to study individual macromolecular components found within all living cells. Through this experiment we will learn the basic sub-units that make up each of these macromolecules while also learning some of their important structural characteristics. This experiment will consist of two parts. The first of which will divide the yeast cells into three of its major macromolecular components: nucleic acids, proteins and polysaccharides. These components are large macromolecules that are quite unique in their composition, structure and function. However, they share a common feature as each macromolecule is composed of repeating subunits, characteristic of the macromolecule. The subunits are linked together by a bond between two adjacent subunits, formed by the loss of water (condensation). Thus, macromolecules can be broken down by the addition of water across the bond, in a process known as hydrolysis. This process was used in the experimental procedure to allow analysis of each individual macromolecule in its subunit form. Proteins are hydrolyzed into amino acids, nucleic acids are hydrolyzed into sugar, base and phosphate, and polysaccharides are broken down into simple sugars...

Words: 728 - Pages: 3

Lab Paper

...Yeast Culture Lab I. Introduction 1. Yeasts are eukaryotic, unicellular fungi that inhabit liquid or moist habitats. They are heterotrophs and rely on complex organic substances for nutrition. Yeasts require oxygen for aerobic cellular respiration but some are also anaerobic with alternative aerobic methods for producing energy. They do not require light to grow and their temperature range varies which means they can survive in a multitude of different environments. Very common, they can be found anywhere, from occurring on the skins of fruits to residing in the intestinal tracts of animals as parasites. The most common method of reproduction for yeasts is asexual reproduction through budding. In this process, the parent cell’s nucleus splits and forms a daughter nucleus. The daughter cell grows on the parent cell until it is large enough to separate. Through cytokinesis, this ‘bud’ then forms a new cell. Less common is the method of sexual reproduction in which spores are formed. In this lab, I will be culturing four yeast samples under different conditions. The goal is to study the effects of limited reproduction, additional resources (nutrition) and predation on the yeast population. Yeasts play a very important role not only by serving as nutrient recyclers in nature, but by also being important for the food industry because they can convert carbohydrates to carbon dioxide and alcohols through fermentation. They are also used in cell biology research and produce ethanol...

Words: 1296 - Pages: 6

Lab 1

...Post-Lab Questions 1. What were your controls for this experiment? What did they demonstrate? Why was saliva included in this experiment? This experiment is looking for amylase in-particular. The first control was a negative control, Water ,this would show there is no Amylase present. The second control would be a Positive control ,the starch solution this would show the presence of the enzyme amylase. The negative control water does not contain starch. Saliva contains Amylase this is why it is included in the experiment. The saliva is a positive because it turned yellow, this shows no starch left because the amylase broke it all down. 2. What is the function of amylase? What does amylase do to starch? The function of amylase is to start the digestion proses. Amylase function is to break down starch to sugar. 3. Which of the foods that you tested contained amylase? Which did not? What experimental evidence supports your claim? The cooked rice contained Amylase and the starch solution contained amylase. The water the Apple contained no starch. 4. Saliva does not contain amylase until babies are two months old. How could this affect an infant’s digestive requirements? Usual babies do not start eating food until three months. The reason there saliva does not contain amylase is because there bodies have not started producing it yet. For there digestion it means they will not break down any starch foods and just pass them trough there......

Words: 630 - Pages: 3

Lab Isolation

...Abstract The purpose of this exercise to become familiar with subtypes of culture media, to learn how to use streak and pour techniques and to generate a pure culture of a specific organism Hypothesis This exercise will allow me to gain an understanding of culture media, to use specific techniques such as streak and pour and generate cultures of specific organisms. Procedures Exercise 1 Part I 1. Disinfect the work area. 2. Melt the agar tubes. 3. Leave the 18 mL tube of MRS agar in hot water (50°C) for use in Part II. 4. Use the marking pencil to label the bottom of one Petri dish S. epidermidis. Pour one half (9mL) of the contents of a tube of nutrient agar into the S. epidermidis Petri dish and the other half into the bottom of an unmarked Petri dish. Cover the dishes and allow them to solidify for use in Part IV. 5. Pour the remaining melted nutrient agar into the unmarked Petri dishes (half a tube per dish). Cover the dishes and allow them to solidify for use in Part III. Part II 1. Disinfect the work area. 2. Label the bottom surface of three sterile Petri dishes L. acidophilus #1, #2, and #3, respectively. 3. Disinfect three test tubes by submerging them in boiling water for 5 minutes. The tubes will be hot, so use tongs or tweezers to lift them out of the water. Be careful not to contaminate the tubes by touching their lips or interiors. When the tubes are cool, label them to match the L. acidophilus Petri dishes. 4. Divide the......

Words: 1233 - Pages: 5

Dna Lab

...single grade. That grade will be included in your Lab Average for the course. Your Lab Average will be included in your final semester average as described in the syllabus for this course. (the deadline for the question submission will be determined in class depending on the progress of the lab) 2. Your scores for Question Sets 4, 5, and 6 will be combined into a single grade. That grade will be included in your Lab Average for the course. (the deadline for the question submission will be determined in class depending on the progress of the lab) 3. You will write a formal lab report in a style and format suitable for submission to the journal Molecular & Cellular Biology. Your report will be included in your final semester average as described in the syllabus for this course. Page 1 of 21 The genetics of S. cerevisiae Biology 2   Introduction For background on yeast, read the Yeast Wiki. Yeast can be grown in the laboratory in two types of media: 1. YPD (yeast peptone dextrose): which contains all necessary nutrients so the yeast do not need to synthesize their own. Auxotrophic yeast strains, which are unable to synthesize all of their own nutrients, will grow on YPD. 2. SD (synthetic dextrose): which does not contain all nutrients so the yeast need to synthesize their own. Specific metabolites (like the amino acids or nitrogenous bases) can be added to the media, when necessary. Prototrophic yeast strains, which can synthesize all of their......

Words: 4873 - Pages: 20

Anaerobic Yeast Fermentation for the Production of Ethanol in a Versatile Lab Fermentor

...Anaerobic yeast fermentation for the production of ethanol in a versatile lab fermentor Abstract Whether used for research or production, the versatile BioFlo® 310 fermentor from New Brunswick Scientific allows growth of a wide variety of aerobic and anaerobic microorganisms, including bacteria, plant, algae, fungi and yeast. Its advanced controller can regulate up to four vessels simultaneously, 120 process loops in all. Here we demonstrate one facet of its versatility—a technique for inducing ethanol production in yeast, by switching from an aerobic growth phase to an anaerobic steady-state culture. Introduction Saccharomyces cerevisiae is a model eukaryotic organism, often used in research because it is easy to manipulate and culture, and is comparatively similar in structure to human cells. This yeast is also widely used in industrial applications to manufacture enzymes and proteins for beer, wine and bread, and because it metabolizes glucose to ethanol, is also used to produce many biofuel products. We produced ethanol from a S. cerevisiae (American Type Culture Collection strain 20602) in a 7.5 liter BioFlo 310 fermentor, to demonstrate the flexibility of this advanced fermentation system. In the first phase, we grew the yeast in an aerobic environment, using a dissolved oxygen cascade control strategy to produce a sufficient cell density. Then we pumped in nitrogen gas to create an anaerobic environment for inducing ethanol production, and used reduction and......

Words: 984 - Pages: 4