Biol 111 Lab Exp.5

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In this experiment we were able to use the technique of Gram staining to differentiate two types of bacteria that were unknown. Gram staining is a method that is used by many scientists in order to identify unknown bacteria’s via the unique physical and chemical characteristics of their cell walls. In a gram test a bacteria can either be gram positive or gram negative. The Gram-positive bacteria have an intricate set of amino sugars which we call peptidoglycan that form a thick cell wall around the plasma membrane. On the other hand, Gram-negative bacteria have an uncomplicated cell wall which thus has less peptidoglycan. The main source of identification within the Gram test is the fact that Gram-positive bacteria appear blue-purple while Gram-negative bacteria appear pink (Gram Stain). Bacteria can come in one of three different shapes; spirilla (spiral-shaped), cocci (round-shaped), and bacilli (rod-shaped). In order to be able to see these shapes a bacteria must be stained or dyed. I hypothesis that we will have one Gram-positive culture and one Gram-negative culture. (Lab Manual)
In order to prepare this lab experiment we performed five steps. The first step in preparing this experiment was to heat fix a bacteria culture sample which in return stopped the sample from coming off the slide during the experiment. In order to perform this first step we first turned on a Bunsen burner and set the flames to a blue color which was used to sterilize an inoculating loop. Next, a drop of water was added to the slide using the loop which was again sterilized via the Bunsen burner. After, bacteria was collected from a culture tube and smeared onto the slide. We spread the bacteria onto the slide until we reached a milky, pale appearance. After we smeared the bacteria we allowed it to air dry and then passed it through the Bunsen burner while holding it with a clothespin…...

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